Primer-site SNPs mask mutations
نویسندگان
چکیده
منابع مشابه
PrimerZ: streamlined primer design for promoters, exons and human SNPs
PrimerZ (http://genepipe.ngc.sinica.edu.tw/primerz/) is a web application dedicated primarily to primer design for genes and human SNPs. PrimerZ accepts genes by gene name or Ensembl accession code, and SNPs by dbSNP rs or AFFY_Probe IDs. The promoter and exon sequence information of all gene transcripts fetched from the Ensembl database (http://www.ensembl.org) are processed before being passe...
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To achieve high-throughput analysis of allele frequencies in human SNPs, we have developed automated methodsfor designing PCR and DNA sequencing primers. We found we could run the PCR assays at quite stringent, uniform conditions. The design process used freely available databases, including dbSNP, SNPper, and TSC, and publicly available software including RepeatMasker and Primer3. We describe ...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2007
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth0307-192